Open AccessRole and regulation of interleukin (IL)-2 receptor alpha and beta chains in IL-2-driven B-cell growth.
Author(s) -
Kenji Nakanishi,
S. Hirose,
Tomohiro Yoshimoto,
Hiromichi Ishizashi,
Keisai Hiroishi,
Toshiyuki Tanaka,
Toru Kono,
M. Miyasaka,
Tadatsugu Taniguchi,
Kazuya Higashino
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.8.3551
Subject(s) - alpha chain , beta (programming language) , receptor , alpha (finance) , interleukin 2 , cell growth , interleukin , biology , interleukin 4 , microbiology and biotechnology , interleukin 15 , b cell , cytokine , chemistry , endocrinology , immunology , antibody , biochemistry , medicine , construct validity , nursing , computer science , patient satisfaction , programming language
Substantial proportions of resting B cells constitutively express low levels of IL-2 receptor (IL-2R) alpha and/or beta chains. The expression of these chains is differentially regulated by anti-IgM and IL-2/IL-4. The anti-IgM induces IL-2R alpha chain expression, whereas each of the two cytokines induces IL-2R beta chain expression in a dose-dependent manner. Moreover, IL-2 induces the growth of B cells, when the cells were pretreated with IL-2 or IL-4 for 24 h. The magnitude of this IL-2-driven B-cell growth depends upon the level of IL-2R beta chain expression. Costimulation of the B cells with IL-2 and anti-IgM shifts the dose-response curve, and the cells proliferate at an IL-2 concentration as low as 40 pM. These results indicate that the levels of anti-IgM-induced IL-2R alpha chain and IL-2-induced IL-2R beta chain determine the sensitivity of the cells to IL-2.