RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains. | Zendy

J. Ribas | Zendy; Reed B. Wickner | Zendy

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RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Author(s) -

J. Ribas,

Reed B. Wickner

Publication year - 1992

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.89.6.2185

Subject(s) - polymerase , biology , rna dependent rna polymerase , rna , rna polymerase i , rna polymerase , genetics , rna editing , small nuclear rna , microbiology and biotechnology , gene

The L-A double-stranded RNA virus of Saccharomyces cerevisiae makes a gag-pol fusion protein by a -1 ribosomal frameshift. The pol amino acid sequence includes consensus patterns typical of the RNA-dependent RNA polymerases (EC 2.7.7.48) of (+) strand and double-stranded RNA viruses of animals and plants. We have carried out "alanine-scanning mutagenesis" of the region of L-A including the two most conserved polymerase motifs, SG...T...NT..N (. = any amino acid) and GDD. By constructing and analyzing 46 different mutations in and around the RNA polymerase consensus regions, we have precisely defined the extent of domains and specific residues essential for viral replication. Assuming that this highly conserved region has a common secondary structure among different viruses, we predict a largely beta-sheet structure.

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