Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: a model for gene therapy. | Zendy

Carmel M. Lynch | Zendy; M M Clowes | Zendy; William Osborne | Zendy; A W Clowes | Zendy; A. Dusty Miller | Zendy

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Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: a model for gene therapy.

Author(s) -

Carmel M. Lynch,

M M Clowes,

William Osborne,

A W Clowes,

A. Dusty Miller

Publication year - 1992

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.89.3.1138

Subject(s) - adenosine deaminase , genetic enhancement , adenosine , vascular smooth muscle , gene , amp deaminase , biology , transfection , microbiology and biotechnology , gene expression , recombinant dna , cell culture , endocrinology , biochemistry , smooth muscle , genetics

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.

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