Open AccessAmplifiable messenger RNA.
Author(s) -
Ying Wu,
David Yong Zhang,
Fred Russell Kramer
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.24.11769
Subject(s) - rna dependent rna polymerase , rna , five prime cap , biology , microbiology and biotechnology , chloramphenicol acetyltransferase , messenger rna , nuclease protection assay , rna editing , non coding rna , polynucleotide , small nuclear rna , rna polymerase , biochemistry , gene expression , gene , reporter gene
RNA molecules were prepared that consisted of an mRNA encoding chloramphenicol acetyltransferase embedded within the sequence of midivariant RNA, which is a template for the RNA-directed RNA polymerase Q beta replicase. These recombinant RNAs were shown to be bifunctional: they are amplified exponentially by incubation with Q beta replicase, and the replicated RNA serves as template for the cell-free synthesis of enzymatically active chloramphenicol acetyltransferase. The availability of amplifiable mRNAs will enable relatively large amounts of protein to be synthesized in vitro.