Open AccessYeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.
Author(s) -
Herbert Tschochner,
Michael H. Sayre,
Peter M. Flanagan,
William J. Feaver,
Roger D. Kornberg
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.23.11292
Subject(s) - transcription factor ii d , transcription factor ii f , transcription factor ii b , taf4 , rna polymerase ii , transcription factor ii e , rna polymerase ii holoenzyme , general transcription factor , biology , termination factor , sigma factor , microbiology and biotechnology , rna polymerase i , transcription factor , taf2 , rna polymerase , transcription (linguistics) , promoter , genetics , rna , gene , gene expression , linguistics , philosophy
Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.