Competitive adsorption of human immunoglobulin G and albumin: consequences for structure and reactivity of the adsorbed layer. | Zendy

Elisabeth Lutanie | Zendy; J.C. Voegel | Zendy; Pierre Schaaf | Zendy; Monique Freund | Zendy; JeanPierre Cazenave | Zendy; A. Schmitt | Zendy

Open Access

Competitive adsorption of human immunoglobulin G and albumin: consequences for structure and reactivity of the adsorbed layer.

Author(s) -

Elisabeth Lutanie,

J.C. Voegel,

Pierre Schaaf,

Monique Freund,

JeanPierre Cazenave,

A. Schmitt

Publication year - 1992

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.89.20.9890

Subject(s) - adsorption , chemistry , polyclonal antibodies , immunoglobulin g , reactivity (psychology) , human serum albumin , albumin , chromatography , serum albumin , antibody , biophysics , biochemistry , organic chemistry , immunology , biology , medicine , alternative medicine , pathology

The affinity of polyclonal anti-IgG for human IgG adsorbed on silica surfaces was investigated by two complementary techniques, scanning angle reflectometry and 125I radiotracing. Special attention was paid to compare the reactivity of IgG adsorbed directly or by exchange with already adsorbed albumin. In particular it was shown that (i) in the first case (direct adsorption) the reaction between anti-IgG and adsorbed IgG was in the ratio 1:1 and (ii) in the second case (adsorption by exchange) there was no reaction.

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