Open AccessMetal-binding chimeric antibodies expressed in Escherichia coli.
Author(s) -
Jaymie R. Sawyer,
Philip W. Tucker,
Frederick R. Blattner
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.20.9754
Subject(s) - periplasmic space , escherichia coli , immunogenicity , metallothionein , hapten , antibody , chemistry , antigen , microbiology and biotechnology , biology , biochemistry , immunology , gene
Metallothionein, a well-characterized biological chelator of metals, has been genetically fused to the binding domain of an antibody and expressed in the periplasm of Escherichia coli. Specific delivery of 109Cd to immobilized hapten or to haptenated cells was demonstrated directly in periplasmic extracts. This approach is potentially useful for targeted radiotherapy and diagnostic imaging. We find six to seven atoms of metal per active antigen-combining site. Absence of the Fc portion of the immunoglobulin along with low immunogenicity of metallothionein-metal complexes should reduce immunologic reactions.