Open AccessIndependent deposition of heterogeneous nuclear ribonucleoproteins and small nuclear ribonucleoprotein particles at sites of transcription.
Author(s) -
S A Amero,
Gopa Raychaudhuri,
Cynthia L. Cass,
W J van Venrooij,
W J Habets,
Adrian R. Krainer,
Ann L. Beyer
Publication year - 1992
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.18.8409
Subject(s) - ribonucleoprotein , small nuclear ribonucleoprotein , snrnp , rna splicing , heterogeneous ribonucleoprotein particle , biology , drosophila melanogaster , cell nucleus , polytene chromosome , microbiology and biotechnology , precursor mrna , heterogeneous nuclear ribonucleoprotein , messenger rna , rna , genetics , gene , nucleus
The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction, or heterogeneous nuclear RNPs (hnRNPs), which traditionally have been thought to function in general pre-mRNA packaging. We obtained antibodies that recognize these two classes of RNP in Drosophila melanogaster. Using a sequential immunostaining technique to compare directly the distribution of these RNPs on Drosophila polytene chromosomes, we found that the two patterns were very similar qualitatively but not quantitatively, arguing for the independent deposition of the two RNP types and supporting a role for hnRNP proteins, but not snRNPs, in general transcript packaging.
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