Open AccessInduction of tolerance to an IgG autoantibody.
Author(s) -
Daniel Offen,
Linda Spatz,
Hal Escowitz,
Stephen M. Factor,
Betty Diamond
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.17.8332
Subject(s) - transgene , biology , autoantibody , genetically modified mouse , allelic exclusion , antibody , immune tolerance , microbiology and biotechnology , immunology , antigen , gene , genetics , immune system , t cell , t cell receptor
Nonautoimmune mice transgenic for the heavy chain of an IgG2b anti-double-stranded-DNA antibody express the transgene in lymphoid organs and display partial allelic exclusion of this gamma 2b transgene. The spleens of these mice are characterized by marked B-cell depletion. Although there are B cells in these mice that express the transgene and recognize double-stranded DNA, they are anergic in vivo. Recovery from the state of anergy occurs in vitro after lipopolysaccharide stimulation. Thus this transgenic model demonstrates the induction of self tolerance to an IgG autoantibody.