Open AccessHigh rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis.
Author(s) -
Christoph G. Grevelding,
Detlef Becker,
Reinhard Kunze,
Albrecht von Menges,
Verena Fantes,
Jeff Schell,
Robert Masterson
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.13.6085
Subject(s) - insertional mutagenesis , transposase , transposition (logic) , cauliflower mosaic virus , biology , genetics , arabidopsis , gene , transposable element , coding region , plasmid , microbiology and biotechnology , mutant , transgene , genetically modified crops , linguistics , philosophy
Overexpression of the Activator (Ac) transposase gene in Arabidopsis thaliana resulted in a minimal germinal transposition frequency of 27% in which independent Dissociation (Ds) transposition events were observed. Molecular analysis of 45 F1 generation Ac/Ds plants indicated that high rates of somatic excision had occurred, and independent germinal insertions were identified in F2 generation progeny plants. A tandem cauliflower mosaic virus (CaMV) promoter fused to two different Ac coding sequences significantly increased the rate of Ds transposition. The CaMV-Ac fusions activated single and multiple copies of two different Ds elements, DsDHFR and Ds35S-1, and reciprocal crosses resulted in similar transposition frequencies. The improved rate of independent germinal transposition observed makes Arabidopsis an ideal system for insertional mutagenesis.