Open AccessThe rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair.
Author(s) -
Gretchen Jimenez,
Jennifer K. Yucel,
Roy Rowley,
Suresh Subramani
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.11.4952
Subject(s) - schizosaccharomyces pombe , g2 m dna damage checkpoint , chek1 , dna repair , schizosaccharomyces , biology , cell cycle checkpoint , dna damage , mutant , dna , mitosis , cell cycle , microbiology and biotechnology , genetics , gene
A number of important molecular checkpoints are believed to control the orderly progression of cell cycle events. We have found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions. Unlike wild-type cells, the mutant cells are unable to arrest in the G2 phase of the cell cycle after DNA damage by gamma-irradiation and are also incapable of maintaining the dependence of mitosis upon the completion of DNA synthesis. An S. pombe genomic clone that complements the UV sensitivity of the rad3-136 mutant completely restores the missing checkpoint functions. The rad3+ gene is also likely to play a role in DNA repair.