Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain. | Zendy

Kevin N. Morris | Zendy; Ira G. Wool | Zendy

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Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.

Author(s) -

Kevin N. Morris,

Ira G. Wool

Publication year - 1992

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.89.11.4869

Subject(s) - depurination , ricin , ribosome , biochemistry , ribosome inactivating protein , amino acid , chemistry , rna , ribosomal rna , protein biosynthesis , hydrolysis , biology , dna , gene , toxin

The A chain of ricin is a RNA N-glycosidase that inactivates ribosomes by depurination of a single adenosine in 28S rRNA. Of the 267 amino acids in the protein, 222 (83%) could be deleted from one or another of 74 mutants without loss of the capacity of the protein to recognize a single nucleotide from among the 7000 in ribosomes or to catalyze hydrolysis.

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