Open AccessDodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.
Author(s) -
José P. Abad,
Mar Carmena,
Sigrid Baars,
Robert D. C. Saunders,
David M. Glover,
P. Ludeña,
C. Sentís,
Chris TylerSmith,
Alfredo Villasanté
Publication year - 1992
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.10.4663
Subject(s) - centromere , satellite dna , biology , minichromosome , heterochromatin , drosophila melanogaster , genetics , euchromatin , genome , chromosome , homo sapiens , satellite , repeated sequence , autosome , gene , sociology , anthropology , engineering , aerospace engineering
To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
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