Open AccessYeast casein kinase I homologues: an essential gene pair.
Author(s) -
Lucy C. Robinson,
E. Jane Albert Hubbard,
Paul R. Graves,
Anna A. DePaoliRoach,
Peter J. Roach,
Ching Kung,
David W. Haas,
Curt H. Hagedorn,
Mark G. Goebl,
Michael R. Culbertson
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.1.28
Subject(s) - casein kinase 1 , casein kinase 2 , gene , biology , biochemistry , saccharomyces cerevisiae , casein kinase 2, alpha 1 , casein , kinase , protein kinase a , cyclin dependent kinase 7 , yeast , cyclin dependent kinase 4 , mitogen activated protein kinase kinase , microbiology and biotechnology , genetics
We report the isolation of an essential pair of Saccharomyces cerevisiae genes that encode protein kinase homologues. The two genes were independently isolated as dosage-dependent suppressors. Increased dosage of YCK1 suppressed defects caused by reduced SNF1 protein kinase activity, and increased dosage of YCK2 relieved sensitivity of wild-type cells to salt stress. The two genes function identically in the two growth assays, and loss of function of either gene alone has no discernible effect on growth. However, loss of function of both genes results in inviability. The two predicted protein products share 77% overall amino acid identity and contain sequence elements conserved among protein kinases. Partial sequence obtained for rabbit casein kinase I shares 64% identity with the two yeast gene products. Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues.