Open AccessCloning of a leucine-zipper protein recognized by the sera of patients with antibody-associated paraneoplastic cerebellar degeneration.
Author(s) -
Hassan M. FathallahShaykh,
Susan C. Wolf,
Edmund Wong,
Jerome B. Posner,
Henry Furneaux
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.8.3451
Subject(s) - leucine zipper , paraneoplastic cerebellar degeneration , complementary dna , open reading frame , cerebellar degeneration , fusion protein , antibody , biology , cerebellum , zinc finger , cloning (programming) , microbiology and biotechnology , gene , peptide sequence , genetics , transcription factor , neuroscience , recombinant dna , autoantibody , computer science , programming language
Antibody-associated paraneoplastic cerebellar degeneration (the Yo syndrome) is an uncommon disorder in which an immune response is specifically directed against tumor tissue and the cerebellum. Screening of a lambda expression library has resulted in the isolation of cDNA clones that encode the major antigen recognized by serum from these patients. The fusion protein produced by the cDNA clones provides the basis of a simple diagnostic assay for this neurological syndrome. The occurrence of leucine-zipper and zinc-finger motifs in the predicted open reading frame suggests that this protein plays a role in the regulation of gene expression.