Open AccessThe arflike gene encodes an essential GTP-binding protein in Drosophila.
Author(s) -
John W. Tamkun,
Richard Kahn,
Mark Kissinger,
Brenda J. Brizuela,
C Rulka,
Matthew P. Scott,
James A. Kennison
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.8.3120
Subject(s) - biology , gene , subfamily , locus (genetics) , genetics , gtp' , gtp binding protein regulators , drosophila melanogaster , recombinant dna , g protein , biochemistry , signal transduction , enzyme
We have identified a Drosophila gene (arflike, arl) encoding a protein that is structurally related (approximately 55% identity) to the ADP-ribosylation factors (ARFs) of yeast and mammals. Biochemical analyses of purified recombinant arl-encoded protein revealed properties similar to the ARF proteins, including the ability to bind and hydrolyze GTP. Clear functional differences between arl and ARF proteins, including a complete lack of ARF activity, suggest that arl is not a functional homolog of ARF. A recessive lethal arl mutation was recovered, demonstrating that the arl locus is an essential gene. We conclude that the arl locus encodes an essential member of the ARF subfamily of small GTP-binding proteins in Drosophila.