Open AccessUltrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis.
Author(s) -
Jinping Liu,
Osamu Shirota,
Donald Wiesler,
Miloš V. Novotný
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.6.2302
Subject(s) - monosaccharide , capillary electrophoresis , fetuin , chemistry , reductive amination , chromatography , galactose , fluorescence , glycoprotein , derivatization , amino sugar , electrophoresis , hexosamines , laser induced fluorescence , derivative (finance) , biochemistry , mass spectrometry , physics , quantum mechanics , financial economics , economics , glucosamine , catalysis
Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide solutes were assessed at low attomole levels (0.5 amol for the CBQCA derivative of galactose). The system has shown considerable promise for high-sensitivity analysis of both neutral and amino sugars in glycoproteins. Complex oligosaccharides, isolated from bovine fetuin by hydrazinolysis, were also successfully "mapped."