Open AccessDNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
Author(s) -
Michael A. White,
Monika Wierdl,
Peter J. Detloff,
Thomas D. Petes
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.21.9755
Subject(s) - saccharomyces cerevisiae , recombination , genetics , flp frt recombination , meiosis , homologous recombination , mitotic crossover , locus (genetics) , genetic recombination , biology , yeast , gene , dna , rap1 , gene conversion
In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.