Open AccessA homeodomain protein binds to gamma-globin gene regulatory sequences.
Author(s) -
Donald Lavelle,
Janice Ducksworth,
Eva M. Eves,
Georgina Gomes,
Michael J. Keller,
Paul Heller,
Joseph DeSimone
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.16.7318
Subject(s) - biology , enhancer , microbiology and biotechnology , locus control region , nuclear protein , gene , emx2 , promoter , enhancer rnas , complementary dna , fusion protein , homeobox , genetics , gene expression , transcription factor , recombinant dna
Developmental regulation of gamma-globin gene expression probably occurs through developmental-stage-specific trans-acting factors able to promote the interaction of enhancer elements located in the far upstream locus control region with regulatory elements in the gamma gene promoters and 3' A gamma enhancer located in close proximity to the genes. We have detected a nuclear protein in K562 and baboon fetal bone marrow nuclear extracts capable of binding to A+T-rich sequences in the locus control region, gamma gene promoter, and 3' A gamma enhancer. SDS/polyacrylamide gel analysis of the purified K562 binding activity revealed a single protein of 87 kDa. A K562 cDNA clone was isolated encoding a beta-galactosidase fusion protein with a DNA binding specificity identical to that of the K562/fetal bone marrow nuclear protein. The cDNA clone encodes a homeodomain homologous to the Drosophila antennapedia protein.