Open AccessDirect proliferative actions of stem cell factor on murine bone marrow cells in vitro: effects of combination with colony-stimulating factors.
Author(s) -
Donald Metcalf,
Nicos A. Nicola
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.14.6239
Subject(s) - stem cell factor , stem cell , progenitor cell , biology , bone marrow , granulocyte macrophage colony stimulating factor receptor , colony stimulating factor , clone (java method) , granulocyte , immunology , precursor cell , microbiology and biotechnology , growth factor , granulocyte macrophage colony stimulating factor , in vitro , haematopoiesis , macrophage , receptor , macrophage colony stimulating factor , cytokine , biochemistry , dna
Stem cell factor (SCF), the ligand for the c-kit protooncogene product, was able to stimulate blast cell and granulocytic colony formation by precursors from normal murine bone marrow. The blast cell colonies contained a high content of progenitor cells able to form macrophage and/or granulocyte colonies. Clone transfer studies, the secondary culture of colony cells, and the culture of populations freed of accessory cells all indicated a direct proliferative action of SCF. SCF receptors were present in high numbers on blast cells and in lower numbers on immature granulocytic, monocytic, and eosinophilic cells. Combination of SCF with granulocyte, granulocyte-macrophage, or multipotential colony-stimulating factors, but not macrophage colony-stimulating factor, resulted in enhancement of colony size. Granulocyte colony-stimulating factor enhanced cell proliferation initiated by SCF, but not vice-versa, and resulted in a 10-fold increase in colony cell numbers and a 7-fold increase in progenitor cells in blast colonies. No evidence was obtained that SCF, alone or in combination with granulocyte colony-stimulating factor, could stimulate self-generation by blast colony-forming cells.