Open AccessCloning of a phospholipase A2-activating protein.
Author(s) -
Mike A. Clark,
Lynne E. Özgür,
Theresa M. Conway,
Janice H. Dispoto,
Stanley T. Crooke,
John S. Bomalaski
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.12.5418
Subject(s) - phospholipase a2 , cloning (programming) , microbiology and biotechnology , leukotriene c4 , leukotriene , messenger rna , phospholipase a , biology , phospholipase , chemistry , enzyme , biochemistry , gene , immunology , asthma , computer science , programming language
Recently we have described the isolation and biochemical characterization of a phospholipase A2-activating protein (PLAP). We have cloned this protein and found it to be expressed as a 2.5-kilobase mRNA. The steady-state levels of PLAP mRNA are induced in smooth muscle and endothelial cells following treatment with leukotriene D4. The increased message levels coincide with increased amounts of PLAP. Synthetic antisense DNA was used to block the synthesis of PLAP and this treatment effectively blocked the activation of phospholipase A2 and the increased generation of prostanoids in smooth muscle and endothelial cells treated with leukotriene D4.