Open AccessMolecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.
Author(s) -
Nobuyuki Kato,
Makoto Hijikata,
Yuko Ootsuyama,
M Nakagawa,
Shogo Ohkoshi,
T Sugimura,
Kunitada Shimotohno
Publication year - 1990
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.24.9524
Subject(s) - hepatitis c virus , virology , biology , nucleic acid sequence , genome , virus , genetics , flaviviridae , open reading frame , complementary dna , peptide sequence , gene
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed.
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