Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. | Zendy

Kristi R. Harkins | Zendy; Richard Jefferson | Zendy; Tony A. Kavanagh | Zendy; Michael W. Bevan | Zendy; David W. Galbraith | Zendy

Open Access

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.

Author(s) -

Kristi R. Harkins,

Richard Jefferson,

Tony A. Kavanagh,

Michael W. Bevan,

David W. Galbraith

Publication year - 1990

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.87.2.816

Subject(s) - protoplast , biology , gene , transgene , transfection , cell sorting , gene expression , chimeric gene , ectopic expression , promoter , nicotiana tabacum , microbiology and biotechnology , reporter gene , genetically modified crops , cell , genetics

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

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