Open AccessConservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis.
Author(s) -
Paul Brown,
Paweł P. Liberski,
Ändy Wolff,
D. Carleton Gajdusek
Publication year - 1990
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.18.7240
Subject(s) - scrapie , infectivity , hamster , proteinase k , gel electrophoresis , biology , polyacrylamide gel electrophoresis , biochemistry , nucleic acid , microbiology and biotechnology , amyloid (mycology) , enzyme , virology , virus , prion protein , medicine , botany , disease , pathology
Infectious extracts of scrapie-infected hamster brain enriched for scrapie-associated fibrils and scrapie amyloid protein (PrP) were partially denatured and subjected to either polyacrylamide gel electrophoresis with subsequent isolation of the PrP band or sequential enzymatic digestion with deglycosidase, phospholipase, proteinase, and several different nucleases. Infectivity measurements of these various specimens revealed a convincing association between infectivity and scrapie amyloid protein, with or without its sugar chains and disulfide bonds, and did not support the hypothesis that nucleic acid is involved in replication.