Biosynthesis of selenium-modified tRNAs in Methanococcus vannielii. | Zendy

M Politino | Zendy; LiKai Tsai | Zendy; Zsolt Veres | Zendy; T C Stadtman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Biosynthesis of selenium-modified tRNAs in Methanococcus vannielii.

Author(s) -

M Politino,

LiKai Tsai,

Zsolt Veres,

T C Stadtman

Publication year - 1990

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.87.16.6345

Subject(s) - selenocysteine , selenium , methanococcus , biochemistry , chemistry , transfer rna , biosynthesis , amino acid , nucleoside , stereochemistry , macromolecule , organic chemistry , rna , escherichia coli , enzyme , gene , cysteine

Selenium-containing nucleosides are natural components of several tRNA species in Methanococcus vannielii. In the present study, the incorporation of selenium from 75SeO3(2-) into these macromolecules was investigated in sonic extracts of M. vannielii. Nucleoside analysis of the 75Se-labeled tRNAs from these in vitro reaction mixtures demonstrated that the selenium was present in 75Se-labeled nucleosides identical to the two naturally occurring 2-selenouridines produced in vivo. Incorporation of selenium into these nucleosides was ATP-dependent and was maximal after 20 min. Addition of O-acetylserine enhanced the activity 2- to 3-fold, implicating a role for selenocysteine in the reaction. Added L-selenocysteine could function as a selenium donor, but the D isomer and DL-selenomethionine were inactive. RPC-5 chromatography of bulk tRNA isolated from M. vannielii grown on 75SeO3(2-) separated five major species of seleno-tRNAs. The amino acid-accepting activity of these tRNAs was investigated.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research