Open AccessGenetic linkage map of 46 DNA markers on human chromosome 16.
Author(s) -
Tim P. Keith,
P Green,
Stephen T. Reeders,
Valerie A. Brown,
P. Phipps,
Angela L. Bricker,
Kathleen Falls,
Kenneth Rediker,
Jody A. Powers,
Christopher Hogan
Publication year - 1990
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.15.5754
Subject(s) - centimorgan , genetics , genetic linkage , locus (genetics) , biology , genetic marker , restriction fragment length polymorphism , gene mapping , complete linkage , recombination fraction , genetic linkage map , chromosome , single nucleotide polymorphism , gene , polymerase chain reaction , genotype
We have constructed a genetic linkage map of human chromosome 16 based on 46 DNA markers that detect restriction fragment length polymorphisms. Segregation data were collected on a set of multigenerational families provided by the Centre d'Etude du Polymorphisme Humain, and maps were constructed using recently developed multipoint analysis techniques. The map spans 115 centimorgans (cM) in males and 193 cM in females. Over much of the chromosome there is a significantly higher frequency of recombination in females than males. Near the alpha-globin locus on the distal part of the short arm, however, there is a significant excess of male recombination. Twenty-seven (59%) of the markers on the map have heterozygosities greater than or equal to 0.50. The largest interval between loci on the sex-average map is 14 cM and the average marker spacing is 3 cM. Using loci on this map, one could detect linkage to a dominant disease on chromosome 16 with as few as 10-15 phase-known meioses.