Open AccessProtection of mice against tumor growth by immunization with an oncogene-encoded growth factor.
Author(s) -
Daniela Talarico,
Michael Ittmann,
Andrea Balsari,
P Delli-Bovi,
Ross S. Basch,
Claudio Basilico
Publication year - 1990
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.11.4222
Subject(s) - oncogene , autocrine signalling , fibroblast growth factor , biology , antibody , microbiology and biotechnology , immunization , amino acid , growth factor , cancer research , oncogene proteins , cell culture , immunology , cell , gene , cell cycle , biochemistry , regulation of gene expression , genetics , receptor
The K-fgf/hst oncogene encodes a growth factor of the fibroblast growth factor (FGF) family that is secreted and transforms cells through a mechanism of autocrine cell proliferation. K-fgf-transformed cells are highly tumorigenic in immunocompetent allogeneic and syngeneic animals. BALB/c mice were immunized with a bacterial fusion protein consisting of a portion of the MS2 polymerase and of the human K-FGF precursor lacking only the first 4 amino acids or with a recombinant protein corresponding to the mature, secreted form of K-FGF (176 amino acids). They were then challenged with syngeneic K-fgf- or H-ras-transformed cells. Vaccinated animals exhibited a significant degree of protection against tumor induction, which was specific for K-fgf-transformed cells and correlated with the ability of the immunized mice to produce high titers of anti-K-FGF antibodies. Thus immunization with a single oncogene product can protect animals against tumor cells expressing this oncogene.