Open AccessCorn metabolites affect growth and virulence of Agrobacterium tumefaciens.
Author(s) -
Shivendra V. Sahi,
Mary-Dell Chilton,
William Scott Chilton
Publication year - 1990
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.10.3879
Subject(s) - agrobacterium tumefaciens , acetosyringone , virulence , biology , plasmid , transformation (genetics) , rhizobiaceae , microbiology and biotechnology , botany , biochemistry , gene , bacteria , genetics , symbiosis
Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 microM AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.
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