Open AccessOn-line analysis of penicillin blood levels in the live rat by combined microdialysis/fast-atom bombardment mass spectrometry.
Author(s) -
Richard M. Caprioli,
ShenNan Lin
Publication year - 1990
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.87.1.240
Subject(s) - fast atom bombardment , mass spectrometry , microdialysis , chemistry , penicillin , pharmacokinetics , tandem mass spectrometry , chromatography , analytical chemistry (journal) , antibiotics , pharmacology , medicine , biochemistry , extracellular
The combination of microdialysis and fast-atom bombardment mass spectrometry has been used to follow the pharmacokinetics of penicillin G directly in the blood-stream of a live rat. After the intramuscular injection of the antibiotic, the blood dialysate was allowed to flow into the mass spectrometer via the continuous-flow/fast-atom bombardment interface. Tandem mass spectrometry provided the means for isolating and recording the ion fragments produced from the drug as the dialysate was exposed to the ionization process. The pharmacokinetic results obtained are compared with those previously reported in the literature, and this on-line method is discussed in terms of its particular advantages.