Open AccessMajor histocompatibility complex class I-restricted activation of cloned T cells by a soluble protein in the absence of accessory cells.
Author(s) -
Thomas Dick,
Angelika B. ReskeKunz
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.7.2316
Subject(s) - major histocompatibility complex , biology , antigen , clone (java method) , antigen presenting cell , t cell , lymphokine , microbiology and biotechnology , mhc restriction , monoclonal antibody , cytotoxic t cell , mhc class i , antibody , immunology , immune system , in vitro , genetics , dna
A T-cell clone, 10BK.1, was established from the draining lymph nodes of (B10 x B10.BR)F1 mice immunized with ovalbumin (OVA) according to standard protocols. Upon coculture with the antigen, 10BK.1 cells reacted by production of lymphokines and by proliferation despite the absence of additional antigen-presenting cells. These T cells do not express major histocompatibility complex (MHC) class II molecules on the cell surface as assessed on the basis of several criteria: by cytofluorometric analysis I-A and I-E determinants were not detectable; 10BK.1 cells could not act as antigen-presenting cells for long-term-cultured MHC class II-restricted T-cell clones; and monoclonal antibodies directed at both MHC class II isotypic complexes (I-A, I-E) did not suppress their OVA-induced activation. In contrast, proliferation of 10BK.1 T cells in response to OVA was abrogated by antibodies directed at H-2Kb antigens. Inhibition experiments employing antibodies directed at Lyt-2 and L3T4 antigens in addition to cytofluorometric analysis revealed that T-cell clone 10BK.1 exhibits the Thy-1+,Lyt-2+,Ly-1-,L3T4- phenotype. 10BK.1 cells pulsed with OVA and fixed with glutaraldehyde induced proliferation of untreated 10BK.1 cells. These data support the theory that 10BK.1 T cells present the exogenous globular protein OVA to one another in an MHC class I-restricted manner, resulting in cell activation and proliferation independent of added accessory cells.