Open AccessDNA binding specificity of the Arc and Mnt repressors is determined by a short region of N-terminal residues.
Author(s) -
Kendall L. Knight,
Robert Sauer
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.3.797
Subject(s) - arc (geometry) , repressor , dna , terminal (telecommunication) , genetics , dna binding protein , chemistry , biology , microbiology and biotechnology , gene , gene expression , transcription factor , computer science , mathematics , telecommunications , geometry
The Arc and Mnt repressors of phage P22 are related proteins that bind to different operator DNA sites. By creating a hybrid Arc-Mnt protein, we show that the binding specificity of Mnt can be switched to that of Arc by replacing six residues at the N terminus of Mnt with the corresponding nine residues from Arc.