Open AccessDopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells.
Author(s) -
Björn Meister,
Jessica Fryckstedt,
Martin Schalling,
Roser Cortés,
Tomas Hökfelt,
Anita Aperia,
Hugh C. Hemmings,
Angus C. Nairn,
Michelle E. Ehrlich,
Paul Greengard
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.20.8068
Subject(s) - dopamine , medicine , endocrinology , dopamine receptor d1 , agonist , dopamine receptor , phosphoprotein , chemistry , protein kinase a , distal convoluted tubule , biology , microbiology and biotechnology , receptor , kidney , phosphorylation , biochemistry , reabsorption
The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein of Mr 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.