Open AccessMonoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells.
Author(s) -
Hideo Yagita,
Toshio Nakamura,
Hajime Karasuyama,
Ko Okumura
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.2.645
Subject(s) - monoclonal antibody , microbiology and biotechnology , immunoprecipitation , immunofluorescence , antibody , complementary dna , biology , western blot , antigen , blot , gene , immunology , biochemistry
Monoclonal antibodies specific for the murine CD2 antigen were identified by an efficient screening method utilizing murine CD2 cDNA transfectants. An unexpected expression of CD2 on murine B cells was revealed by immunofluorescence and immunoprecipitation studies with these monoclonal antibodies and by RNA blot analysis for the murine CD2 transcript.