Interaction of cellular factors related to the Jun oncoprotein with the promoter of a replication-dependent hamster histone H3.2 gene. | Zendy

Ajay Sharma | Zendy; Timothy J. Bos | Zendy; A Pekkala-Flagan | Zendy; Peter K. Vogt | Zendy; Amy S. Lee | Zendy

Open Access

Interaction of cellular factors related to the Jun oncoprotein with the promoter of a replication-dependent hamster histone H3.2 gene.

Author(s) -

Ajay Sharma,

Timothy J. Bos,

A Pekkala-Flagan,

Peter K. Vogt,

Amy S. Lee

Publication year - 1989

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.86.2.491

Subject(s) - biology , microbiology and biotechnology , histone , dna replication , promoter , gene , dna , histone h3 , genetics , gene expression

The promoter region of a replication-dependent histone H3.2 gene contains a putative DNA binding site for the Jun oncoprotein within a 32-nucleotide regulatory domain. The hamster sequence differs by one nucleotide from the AP-1 consensus sequence found in several promoters responsive to phorbol 12-myristate 13-acetate. We have identified the factors interacting with this region as 42- and 45-kDa proteins by DNA affinity chromatography, immunoblotting, and UV crosslinking. These proteins, which are candidates for conferring high-level expression to the histone promoter, share an antigenic epitope with the DNA-binding domain of Jun but diverge from it at the amino terminus. The interaction of these proteins with the promoter of a replication-dependent cellular gene provides evidence that members of the Jun oncoprotein family may play specific roles in the expression of genes essential for progression of the cell cycle.

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