Open AccessExpression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.
Author(s) -
Dietrich H. Nies,
Anke Nies,
Lien Chu,
Simón Silver
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.19.7351
Subject(s) - operon , efflux , nucleic acid sequence , plasmid , escherichia coli , biology , alcaligenes , nucleotide , divalent , microbiology and biotechnology , lac operon , biochemistry , chemistry , dna , gene , bacteria , genetics , pseudomonas , organic chemistry
Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.