Open AccessHuman immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro.
Author(s) -
Colin Dingwall,
Ingemar Ernberg,
Michael J. Gait,
Sheila M. Green,
Shaun Heaphy,
Jonathan Karn,
Anthony D. Lowe,
Mandeep Singh,
Michael A. Skinner,
R Di Valerio
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.18.6925
Subject(s) - rna , microbiology and biotechnology , immunoprecipitation , tar (computing) , hiv long terminal repeat , biology , activator (genetics) , in vitro , dna , rna binding protein , gene , chemistry , gene expression , biochemistry , long terminal repeat , computer science , programming language
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.