Open AccessChromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.
Author(s) -
Richard Iggo,
A C Gough,
WeiHua Xu,
David P. Lane,
Nigel K. Spurr
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.16.6211
Subject(s) - microbiology and biotechnology , biology , complementary dna , gene , polymerase chain reaction , dna polymerase , chromosome , southern blot , chromosome 22 , dna , genetics
Chromosome mapping by Southern analysis of DNA from somatic cell hybrids is often unsuccessful when only cDNA probes are available. p68 is a putative RNA helicase that is antigenically related to the simian virus 40 large tumor antigen. By using the polymerase chain reaction and oligode-oxynucleotide primers based on the cDNA sequence, we have identified introns in the p68 gene. Comparison of human and mouse DNA fragments amplified with these primers revealed length differences that allowed us to identify the human gene. Application of this technique to DNA from human-mouse somatic cell hybrids and cell lines derived from them by chromosome-mediated gene transfer allowed us to map p68 to the distal part of the long arm of chromosome 17.