Open AccessDissection of functional domains of adenovirus DNA polymerase by linker-insertion mutagenesis.
Author(s) -
M Chen,
Marshall S. Horwitz
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.16.6116
Subject(s) - polymerase , dna polymerase , dna polymerase mu , dna clamp , dna polymerase ii , biology , microbiology and biotechnology , dna polymerase i , dna , genetics , gene , polymerase chain reaction , circular bacterial chromosome , reverse transcriptase
Linker-insertion mutations were introduced into the cloned adenovirus DNA polymerase gene and the functional effects on the initiation and elongation of DNA in vitro were measured. Essential regions of the polymerase appear to be scattered in patches across the entire molecule and are not limited to the five regions of homology shared with a variety of other replicating polymerases. Thus, the adenovirus DNA polymerase presumably contains active sites that must be formed by distant interactions across the polymerase molecule.