Open AccessDouble-negative (CD4-CD8-) lymphocytes bearing T-cell receptor alpha and beta chains in normal human skin.
Author(s) -
Veronika Groh,
Marina Fabbi,
Frans Hochstenbach,
Richard T. Maziarz,
Jack L. Strominger
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.13.5059
Subject(s) - t cell receptor , cd8 , biology , alpha chain , cd3 , microbiology and biotechnology , t lymphocyte , lymphokine , beta (programming language) , t cell , cytotoxic t cell , immune system , population , receptor , immunology , genetics , in vitro , medicine , environmental health , computer science , programming language
Double-negative (CD4-CD8-) T-cell receptor alpha- and beta-chain (TCR alpha beta)-expressing lymphocytes have been identified in human skin. Two skin-derived lymphocyte cell lines (II-1 and II-2) expressing this phenotype were obtained. They each expressed full-length TCR alpha- and beta-chain transcripts and lacked TCR gamma- and delta-chain as well as CD4 and CD8 mRNAs. These polyclonal cell lines shared one predominant TCR beta-chain gene rearrangement. The CD3/TCR alpha beta complexes were functionally competent as evidenced by their capacity to transduce activation signals resulting in cell proliferation, lymphokine secretion, and cytolytic activity. The demonstration of CD4-CD8-TCR alpha beta lymphocytes as a subpopulation of the skin-associated immune system should contribute to understanding the biologic potential of this cell population as well as of this organ-specific immune system.