Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences. | Zendy

J M Huibregtse | Zendy; David R. Engelke | Zendy; Dennis J. Thiele | Zendy

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Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences.

Author(s) -

J M Huibregtse,

David R. Engelke,

Dennis J. Thiele

Publication year - 1989

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.86.1.65

Subject(s) - metallothionein , yeast , footprinting , biology , transcription factor , saccharomyces cerevisiae , gene , locus (genetics) , microbiology and biotechnology , genetics , biochemistry

Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors. We have used a high-resolution chromosomal footprinting technique to detect the interaction of cellular factors with UASCUP1. Our results demonstrate that one or more cellular factors bind to UASCUP1 in a copper-inducible fashion. This copper-inducible binding is enhanced in a yeast strain that harbors several copies of the positive regulatory gene ACE1 and is not detectable in yeast cells that contain a nonfunctional (ace1-delta 1) locus. The correlation between yeast metallothionein gene activation and copper-inducible occupation of UASCUP1 sequences suggests that the binding of metallothionein transcriptional regulatory factors to cis-acting control sequences is copper-inducible.

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