Open AccessCharacterization and expression of a cDNA encoding the human androgen receptor.
Author(s) -
Wayne D. Tilley,
Marco Marcelli,
Jean D. Wilson,
Michael J. McPhaul
Publication year - 1989
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.86.1.327
Subject(s) - complementary dna , biology , androgen receptor , dihydrotestosterone , mineralocorticoid receptor , rnf4 , microbiology and biotechnology , receptor , glucocorticoid receptor , peptide sequence , androgen , cdna library , mineralocorticoid , biochemistry , hormone , gene , genetics , prostate cancer , cancer
We report the isolation of a cDNA that encodes the complete human androgen receptor. The cDNA predicts a protein of 917 amino acids with a molecular weight of 98,918. Introduction of the cDNA into heterologous mammalian cells caused expression of high levels of a protein that binds dihydrotestosterone with the affinity, specificity, and sedimentation properties characteristic of the native human androgen receptor. Comparisons with the amino acid sequence of previously cloned steroid hormone receptors revealed a high degree of sequence conservation with the progesterone, glucocorticoid, and mineralocorticoid receptors in the putative hormone and DNA-binding domain regions.