Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain. | Zendy

Richard Bond | Zendy; Kess Jansen | Zendy; David I. Gottlieb | Zendy

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Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.

Author(s) -

Richard Bond,

Kess Jansen,

David I. Gottlieb

Publication year - 1988

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.85.9.3231

Subject(s) - glutamate decarboxylase , messenger rna , biology , rna , glutamic acid , nuclease protection assay , microbiology and biotechnology , nuclease , northern blot , enzyme , biochemistry , amino acid , gene , non coding rna

The time and pattern of appearance of glutamic acid decarboxylase (glutamate decarboxylase; EC 4.1.1.15) (GAD) mRNA during the development of the rat brain were analyzed. RNA transfer blot analysis of poly(A)+ RNA from whole brain shows that a 3.7-kilobase transcript is the most abundant form of the message from embryonic day 15 (E15) through adulthood. By E15 this form is present at about 50% of its adult abundance relative to other poly(A)+ mRNA species. At birth the abundance is approximately the same as in the adult. In contrast, the enzyme activity level is only 8% of the adult level at birth and takes 3 weeks to reach adult levels. There are qualitative changes in GAD mRNA during development. Several large (7-9 kilobases) transcripts with strong homology to GAD are enriched in early developmental stages but are barely detectable in the adult. A nuclease protection assay shows a developmentally regulated heterogeneity in a coding portion of the mRNA.

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