Open AccessIsolation and sequence of a cDNA clone encoding the 31-kDa subunit of bovine kidney vacuolar H+-ATPase.
Author(s) -
Sheldon Hirsch,
Arnold W. Strauss,
Kamran Masood,
S Lee,
Vikas P. Sukhatme,
Stephen L. Gluck
Publication year - 1988
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.9.3004
Subject(s) - complementary dna , protein subunit , biology , microbiology and biotechnology , interleukin 10 receptor, alpha subunit , g alpha subunit , gamma aminobutyric acid receptor subunit alpha 1 , messenger rna , biochemistry , gene
We have isolated a cDNA encoding the 31-kDa subunit of the bovine kidney vacuolar H+-ATPase. The composite sequence contains 1219 base pairs, which includes the entire 678-base-pair coding region. A lysine-rich sequence previously found in the Na+, K+-ATPase alpha subunit and the H+,K+-ATPase was identified in the 31-kDa subunit. An RNA blot and an immunoblot demonstrated variable 31-kDa subunit expression and immunoreactivity in different tissues; the highest levels were observed in kidney medulla and brain with both types of analysis. The isolation of a cDNA for the 31-kDa subunit is an important step in understanding this subunit's role in the function and regulation of the vacuolar H+-ATPase.
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