Open AccessSelective differentiation and proliferation of hematopoietic cells induced by recombinant human interleukins.
Author(s) -
Hirohisa Saito,
Kiyohiko Hatake,
Ann M. Dvorak,
Kristin M. Leiferman,
Albert D. Donnenberg,
Naoko Arai,
Kimishige Ishizaka,
Teruko Ishizaka
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.7.2288
Subject(s) - bone marrow , cord blood , haematopoiesis , interleukin 3 , interleukin 5 , peripheral blood mononuclear cell , immunology , biology , interleukin , cytokine , stem cell , microbiology and biotechnology , t cell , interleukin 21 , immune system , in vitro , biochemistry
Effects of recombinant human interleukins on hematopoiesis were explored by using suspension cultures of mononuclear cells of human umbilical-cord blood and bone marrow. The results showed that interleukin 5 induced the selective differentiation and proliferation of eosinophils. After 3 weeks in culture with interleukin 5, essentially all nonadherent cells in both bone marrow and cord blood cell cultures became eosinophilic myelocytes. Culture of the same cells with interleukin 4 resulted in the selective growth of OKT3+ lymphocytes. However, OKT3+ cells did not develop if the bone marrow cells were depleted of OKT3+/OKT11+ cells prior to the culture, indicating that interleukin 4 induced the proliferation of a subpopulation of resting T cells present in cord blood and bone marrow cell preparations. In suspension cultures of bone marrow cells and cord blood cells grown in the presence of interleukin 3, basophilic, eosinophilic, and neutrophilic myelocytes and macrophages developed within 2 weeks. By 3 weeks, however, the majority of nonadherent cells became eosinophilic myelocytes. In contrast to mouse bone marrow cell cultures, neither interleukin 3 nor a combination of interleukins 3 and 4 induced the differentiation of mast cells in human bone marrow or cord blood cell cultures.