Open AccessSuppression of contractile force in muscle fibers by antibody to myosin subfragment 2.
Author(s) -
Stephen J. Lovell,
Trudy Karr,
William F. Harrington
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.6.1849
Subject(s) - myosin , isometric exercise , polyclonal antibodies , antibody , myosin light chain kinase , chemistry , muscle contraction , biophysics , biochemistry , biology , anatomy , immunology , physiology
Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.