Open AccessLigand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts.
Author(s) -
Heimo Riedel,
S. Massoglia,
Joseph Schlessinger,
Axel Ullrich
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.5.1477
Subject(s) - 3t3 cells , receptor , epidermal growth factor , transfection , microbiology and biotechnology , epidermal growth factor receptor , biology , transforming growth factor , growth factor , ligand (biochemistry) , growth factor receptor , transformation (genetics) , cell growth , cancer research , cell culture , chemistry , signal transduction , biochemistry , gene , genetics
The cell surface receptor for the mitogenic peptide epidermal growth factor (EGF) is involved in control of normal cell growth and may play a role in the genesis of human neoplasia such as squamous carcinoma and glioblastoma. Soft-agar growth and focus-formation experiments with NIH 3T3 mouse fibroblasts transfected with an expression plasmid demonstrated the ligand-dependent transforming potential of the human EGF receptor without structural alterations. Activation of overexpressed normal receptor alone appears to be sufficient for transformation of NIH 3T3 cells in vitro.