Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes. | Zendy

G. Pons | Zendy; Cindy Raefsky-Estrin | Zendy; Donna J. Carothers | Zendy; R A Pepin | Zendy; A A Javed | Zendy; B.W. Jesse | Zendy; Mahrukh K. Ganapathi | Zendy; David Samols | Zendy; M S Patel | Zendy

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Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes.

Author(s) -

G. Pons,

Cindy Raefsky-Estrin,

Donna J. Carothers,

R A Pepin,

A A Javed,

B.W. Jesse,

Mahrukh K. Ganapathi,

David Samols,

M S Patel

Publication year - 1988

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.85.5.1422

Subject(s) - dihydrolipoamide dehydrogenase , complementary dna , open reading frame , biology , microbiology and biotechnology , peptide sequence , cdna library , nucleic acid sequence , dehydrogenase , biochemistry , amino acid , protein primary structure , untranslated region , gene , messenger rna , enzyme

cDNA clones comprising the entire coding region for human dihydrolipoamide dehydrogenase (dihydrolipoamide:NAD+ oxidoreductase, EC 1.8.1.4) have been isolated from a human liver cDNA library. The cDNA sequence of the largest clone consisted of 2082 base pairs and contained a 1527-base open reading frame that encodes a precursor dihydrolipoamide dehydrogenase of 509 amino acid residues. The first 35-amino acid residues of the open reading frame probably correspond to a typical mitochondrial import leader sequence. The predicted amino acid sequence of the mature protein, starting at the residue number 36 of the open reading frame, is almost identical (greater than 98% homology) with the known partial amino acid sequence of the pig heart dihydrolipoamide dehydrogenase. The cDNA clone also contains a 3' untranslated region of 505 bases with an unusual polyadenylylation signal (TATAAA) and a short poly(A) track. By blot-hybridization analysis with the cDNA as probe, two mRNAs, 2.2 and 2.4 kilobases in size, have been detected in human tissues and fibroblasts, whereas only one mRNA (2.4 kilobases) was detected in rat tissues.

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