Open AccessSynthesis of phosphatidylethanol--a potential marker for adult males at risk for alcoholism.
Author(s) -
Gerald C. Mueller,
Michael F. Fleming,
Matthew Lemahieu,
G S Lybrand,
Kevin J. Barry
Publication year - 1988
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.24.9778
Subject(s) - phosphatidylethanol , phospholipase d , phorbol , chemistry , alcohol , ethanol , population , endocrinology , enzyme , biochemistry , protein kinase c , medicine , phosphatidylcholine , phospholipid , membrane , environmental health
Phosphatidylethanol, whose synthesis is catalyzed by a phospholipase D in a transphosphatidylation reaction, is a unique metabolite of ethanol. Phorbol 12-tetradecanoate 13-acetate, a tumor-promoting phorbol ester and stimulator of protein kinase C, activates this enzyme in peripheral blood lymphocytes. This system has been developed into an assay for measuring the potential of this pathway in human subjects. A pilot study of phosphatidylethanol synthesis in lymphocytes of adult males who have both an alcohol dependency and a family history of alcoholism has revealed that the average potential for phosphatidylethanol synthesis in this population is significantly elevated over that of control subjects.
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