Open AccessRapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
Author(s) -
Aharon S. Cohen,
Diajarian,
Aran Paulus,
András Guttman,
John A. Smith,
Barry L. Karger
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.24.9660
Subject(s) - capillary electrophoresis , chromatography , oligonucleotide , electrophoresis , gel electrophoresis , chemistry , polyacrylamide gel electrophoresis , free flow electrophoresis , gel electrophoresis of proteins , polyacrylamide , dna , biochemistry , enzyme , polymer chemistry
Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.