Open AccessAntisense ribosomal protein gene expression specifically disrupts oogenesis in Drosophila melanogaster.
Author(s) -
Su Wen Qian,
Seiji Hongō,
Marcelo JacobsLorena
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.24.9601
Subject(s) - drosophila melanogaster , biology , gene expression , gene , genetics , melanogaster , rna , antisense rna , oogenesis , messenger rna , regulation of gene expression , microbiology and biotechnology , embryogenesis
To assess the functional importance of ribosomal protein rpA1 gene expression during development of Drosophila melanogaster, we have transformed into the fly's genome an antisense rpA1 gene driven by a heat shock promoter. Antisense rpA1 expression severely disrupted oogenesis and produced a "small egg" female-sterile phenotype. The severities of these defects were proportional to the level of antisense rpA1 expression. Anti-rpA1 expression did not affect larval or pupal development. Quantitative RNA analysis suggested that high anti-rpA1 expression results in a general decrease of mRNA in the ovary.